4.5 Article

Metabolites of Lactobacillus plantarum 2142 Prevent Oxidative Stress-Induced Overexpression of Proinflammatory Cytokines in IPEC-J2 Cell Line

Journal

INFLAMMATION
Volume 35, Issue 4, Pages 1487-1499

Publisher

SPRINGER/PLENUM PUBLISHERS
DOI: 10.1007/s10753-012-9462-5

Keywords

IPEC-J2; probiotics; oxidative stress; proinflammatory cytokines; Hsp70

Funding

  1. Hungarian Scientific Research Fund [76133, 100701]

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Probiotics have already proven beneficial effects in the treatment of several intestinal infections, but the underlying mechanisms how the probiotics can affect responses of porcine IPEC-J2 enterocytes to oxidative stress remained to be elucidated. The immunmodulatory effect of five bacterial strains (Lactobacillus plantarum 2142, Lactobacillus casei Shirota, Bifidobacterium animalis subsp. lactis BB-12, Bacillus amyloliquefaciens CECT 5940 and Enterococcus faecium CECT 4515) on 1 mM peroxide-triggered upregulation of interleukin (IL)-8 and tumor necrosis factor alpha (TNF-alpha) level was screened by q RT-PCR. Our data revealed that spent culture supernatant (SCS) of L. plantarum 2142 had significant lowering effect on IL-8 and TNF-alpha level with concomitant promoting activity on protective Hsp70 gene expression. According to our results, lactic acid (racemic, d- and l-lactic acid) and acetic acid produced by lactobacilli had no protective effect in quenching upregulation of proinflammatory cytokines. Furthermore, L. plantarum 2142-specific supernatant peptides were detected by gel electrophoresis and capillary zone electrophoresis.

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