Journal
INFLAMMATION
Volume 34, Issue 2, Pages 111-118Publisher
SPRINGER/PLENUM PUBLISHERS
DOI: 10.1007/s10753-010-9214-3
Keywords
cryptotanshinone; lipopolysaccharides; cytokines; NF-kappa B; MAPKs
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Funding
- National Natural Science Foundation of China [30811120434, 30772576]
- National Science and Technology Major Project of China [2009ZX09102-152, 2009ZX09303-007]
- People's Government of Guangdong Province (P. R. of China) [2003A30904]
- Key Natural Science Foundation of Guangdong Province, People's Republic of China [7117380]
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Cryptotanshinone (CTS), a major constituent extracted from the medicinal herb Salvia miltiorrhiza Bunge, has well-documented antioxidative and anti-inflammatory effects. In the present study, the pharmacological effects and underlying molecular mechanisms of CTS on lipopolysaccharide (LPS)-induced inflammatory responses were investigated. By enzyme-linked immunosorbent assay, we observed that CTS reduced significantly the production of proinflammatory mediators (tumor necrosis factor-alpha and interleukin-6) induced by LPS in murine macrophage-like RAW264.7 cells. Mechanistically, CTS inhibited markedly the phosphorylation of mitogen-activated protein kinases (MAPKs), including ERK1/2, p38MAPK, and JNK, which are crucially involved in regulation of proinflammatory mediator secretion. Moreover, immunofluorescence and western blot analysis indicated that CTS abolished completely LPS-triggered nuclear factor-kappa B (NF-kappa B) activation. Taken together, these data implied that NF-kappa B and MAPKs might be the potential molecular targets for clarifying the protective effects of CTS on LPS-induced inflammatory cytokine production in macrophages.
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