Journal
INFECTION GENETICS AND EVOLUTION
Volume 9, Issue 2, Pages 278-282Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.meegid.2008.12.008
Keywords
Congenital Chagas disease; Lineages; Trypanosoma cruzi IId; Multilocus enzyme electrophoresis (MLEE); Random amplified polymorphic DNA (RAPD)
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Funding
- CONICET (National Research Council)
- CIUNSa (Research Council of the National University of Salta)
- European Union [E05D057391AR]
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Trypanosoma cruzi is genetically classified into six discrete phylogenetic lineages on the basis of different genetic markers. Identifying lineages circulating among humans in different areas is essential to understand the molecular epidemiology of Chagas disease. In the present study, 18 T. cruzi isolates from congenitally infected newborns in the northwestern province of Salta-Argentina were studied by multilocus enzyme electrophoresis (MLEE) and random amplified polymorphic DNA (RAPD). All isolates were typed by MLEE and RAPID as belonging to T. cruzi lid. Analysis of minor variants of TcIId using probes hybridizing with hypervariable domains of kDNA minicircles, detected three variants with a similar distribution among the isolates. Our findings confirm the presence of T. cruzi IId among congenitally infected newborns in northwestern Argentina and support the assumption that human infection by T. cruzi in the Southern Cone countries of Latin America is due principally to T. cruzi II. (C) 2008 Elsevier B.V. All rights reserved.
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