Journal
INFECTION AND IMMUNITY
Volume 77, Issue 6, Pages 2568-2575Publisher
AMER SOC MICROBIOLOGY
DOI: 10.1128/IAI.01537-08
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Funding
- Swiss National Science Foundation [3100AQ-105541, 310000-113623/1]
- European Union [032296]
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Lipopolysaccharide (LPS) is a major constituent of the outer membrane and an important virulence factor of Salmonella enterica subspecies 1 serovar Typhimurium (serovar Typhimurium). To evaluate the role of LPS in eliciting intestinal inflammation in streptomycin-treated mice, we constructed an O-antigen-deficient serovar Typhimurium strain through deletion of the wbaP gene. The resulting strain was highly susceptible to human complement activity and the antimicrobial peptide mimic polymyxin B. Furthermore, it showed a severe defect in motility and an attenuated phenotype in a competitive mouse infection experiment, where the Delta wbaP strain (SKI12) was directly compared to wild-type Salmonella. Nevertheless, the Delta wbaP strain (SKI12) efficiently invaded HeLa cells in vitro and elicited acute intestinal inflammation in streptomycin-pretreated mice. Our experiments prove that the presence of complete LPS is not essential for in vitro invasion or for triggering acute colitis.
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