An An economic and green strategy for preventing Chinese medicinal materials from toxigenic fungi: Couplet medicine technique

An economic, safe and environment-friendly strategy for preventing Chinese medicinal materials from fungi and aflatoxins contamination is in great need. This study aimed to develop a couplet medicine technique with low cost, little time and few labors for the first time for scientific preservation of Radix Notoginseng by investigating the inhibitory efficacy of some aromatic Chinese medicinal materials regarding their anti-Aspergillus flavus properties with the mold changes and aflatoxins accumulation as the detection indexes. An ultra-performance liquid chromatography with fluorescence detection method was validated for sensitive determination of four aflatoxins in Radix Notoginseng without or with the effects of protector from the couplet medicines. It was found that Cortex Cinnamomi, Fructus Anisi Stellati, Flos Caryophylli and Cortex Mouton with strong anti-A. flavus activities could be the potential protector medicines to prevent Radix Notoginseng from molds and aflatoxins contamination. After co-storage of one of the four candidates with Radix Notoginseng to form couplet medicine, expected results expressed that Cortex Mouton was the best protector medicine at the weight ratio of protector (Cortex Mouton): protege (Radix Notoginseng) of at least 2:1 (w/w). In practice, the couplet medicine of Cortex Mouton-Radix Notoginseng designed through the embedding and stratified stacking modes could effectively prevent toxigenic fungi contamination and aflatoxins residue in Radix Notoginseng to warrant the quality and safety of this highly-valuable Chinese medicinal material. The green couplet medicine strategy with lower cost and less labor can be preferred for scientific preservation of Chinese medicinal materials, foods and other matrices.