Coriandrum sativum seed extract assisted in situ green synthesis of silver nanoparticle and its anti-microbial activity

Extracellular in situ synthesis of metal nanoparticles using extracts of plants like Azadirachta indica (Neem), and Zingiber officinale (Ginger) has been successfully carried out earlier. In this study we have developed a novel method to synthesize silver nanoparticles by the process of bio-chemical reaction between silver salt solution with seed extract of Coriandrum sativum without using any surfactant or external energy. By this method physiologically stable, bio-compatible Ag nanoparticles (NPs) were formed. These functionalized AgNPs could be used for targeted drug delivery with enhanced therapeutic efficacy and minimal side effects. It is well known that on treating the metallic salt solution with some plant extracts, a rapid reduction occur leading to the formation of highly stable metal nanoparticles. This method resulted in rapid synthesis of nanoparticles, i.e. reaction time was 1-2 h as compared to 2-4 days required by microorganisms. These nanoparticles were analysed by various characterization techniques to reveal their morphology and chemical composition. TEM image of these NPs indicated the formation of spherical, non-uniform, poly dispersed nanoparticles of mean size of 13.09 nm. A detailed study of anti-microbial activity of nanoparticles and its synergetic effectiveness with common narrow spectrum antibiotic has been successfully carried out. (C) 2014 Published by Elsevier B.V.