Preparation of polyamide nanocapsules of Elaeagnus angustifolia L. delivery with in vivo studies

The purpose of this study is to prepare polyamide nanocapsules containing Elaeagnus angustzfolia L using an emulsion diffusion technique with in vivo studies. Diethylenetriamine (DETA) was used as the encapsulating polymer in acetone ethyl acetate (EA) and dimethyl sulfoxide (DMSO) as the organic solvents, Tween 80, Tween 60, Tween 20, Gelatin, and sodium lauryl sulfate (SLS), in water as the stabilizers. Two ratios of organic to aqueous phases were used with each solvent and stabilizer. The nanocapsule E. angustzfolia were obtained with a mean diameter of 60-180 nm, zeta potential of -11.3 mV, Particles Dispersion Index (PDI) of 0.2, and a high encapsulation efficiency of 0.98%. Acetone was superior to EA and DMSO, and Tween 20 was superior to Tween 80, Tween 60, Gelatin and SLS in obtaining smaller nanocapsules. An organic of aqueous phase ratio of 1:5 was shown to be more suitable for the smaller nanocapsules. Finally, nanocapsules containing E. angustifolia and calcium carbonate as well as calcium tablets available on the market, were administered to rats for 3 weeks, and the calcium levels were measured in their blood as well as compared to a normal control group. The results showed that the E. angustifolia group has a higher level of calcium in their blood. (C) 2014 Elsevier B.V. All rights reserved.