Green synthesis and characterization of silver nanoparticles using Boerhaavia diffusa plant extract and their anti bacterial activity

Green synthesis of silver nanoparticles was carried out using Boerhaavia diffuse plant extract as a reducing agent. The biosynthesized AgNPs were characterized by SEM-EDAX, XRD, TEM, UV-Vis, and FT-IR spectroscopy techniques. UV-Vis spectroscopy of prepared silver colloidal solution showed absorption maxima at 418 nm. XRD and TEM analysis revealed that AgNPs are face-centered, cubic structure being spherical in shape with an average particle size of 25 nm. The AgNPs were tested for antibacterial activity against three fish bacterial pathogens viz., Aeromonas hydrophila, Pseudomonas fluorescens and Flavobacterium branchiophilum and they demonstrated highest sensitivity toward F. branchiophilum when compared to other two bacterial pathogens. (C) 2013 Elsevier B.V. All rights reserved.