Green synthesis of silver nanoparticles by Trichoderma harzianum and their bio-efficacy evaluation against Staphylococcus aureus and Klebsiella pneumonia

Silver nanoparticles were successfully synthesized from agriculturally beneficial fungus Trichoderma harzianum through a simple green and eco-friendly route using cell filtrate. The silver nanoparticles formation was evaluated at different temperatures and concentrations of AgNO3. The synthesized nanoscale particles were analyzed by UV-vis spectrum, TEM, XRD, SEM and EDS, which revealed that the synthesised nanoparticles had face centred cubic symmetry. Size range (51.10 nm) and stability of the synthesized silver nanoparticles were evaluated by DLS and found stable for three months. The antibacterial activities of synthesized nanosilver was evaluated against two bacteria; Staphylococcus aureus and Klebsiella aeruginosa and it was found that bacterial growth was significantly reduced in a dose dependent manner. (C) 2014 Elsevier B.V. All rights reserved.