Microsatellite polymorphism in Jatropha curcas L.-A biodiesel plant

We developed and characterized 1207 SSRs to enrich the validated markers repertoire of Jatropha curcas. A total of 248 polymorphic SSRs were identified with a panel of 7 accessions off. curcas including some exotic accessions. Furthermore, 179 and 331 SSRs were found polymorphic among parental lines of NBRI-J05 x EC643912 and Chhatrapati x Jatropha integerrima used in developing two mapping population respectively. The number of alleles varied from 2 to 5 with an average of 2.24 +/- 0.55 and 2.42 +/- 0.62 alleles/SSR for CA and GA enriched library respectively. Most of the SSRs had lower PIC value (less than 0.30) and the maximum PIC value was observed for JGM_A281, JGM_A326 (0.63) followed by JGM_B300 (0.62), JGM_B361 (0.58), JGM_A244 (0.55), JGM_B595 (0.55) and JGM_B176 (0.55). The genetic similarity coefficient among the accessions off. curcas and one accession off. integerrima ranged from 0.11 to 0.92 with an average of 0.57 +/- 0.31. The phenogram classified all the 7 accessions off. curcas in one cluster and the J. integerrima remained as an out group. The BLASTX analysis of SSR containing sequences showed maximum similarity of 50% with Ricinus communis (Euphorbiaceae) followed by Populus trichocarpa (22%), Vitis vinifera (16%) and Arabidopsis spp. (4.5%). This study may enrich the validated repertoire of SSR markers in J. curcas and could be used in various genetic studies including construction of linkage map, diversity analysis, and QTL/association mapping. (C) 2013 Elsevier B.V. All rights reserved.