Journal
MATRIX BIOLOGY
Volume 48, Issue -, Pages 26-35Publisher
ELSEVIER
DOI: 10.1016/j.matbio.2015.08.002
Keywords
Perlecan; Muscle atrophy; Autophagy; Mechanical stress; Soleus muscle
Categories
Funding
- NIDCR [DE000185-27]
- Japan Agency for Medical Research and Development (AMED) [15ek0109017s0202]
- Intramural Research Grant (26-8) for Neurological and Psychiatric Disorders of NCNP [26-8]
- MEXT [S1101009 2011-2015]
- Grants-in-Aid for Scientific Research [15K09326] Funding Source: KAKEN
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The autophagy lysosome system is essential for muscle protein synthesis and degradation equilibrium, and its dysfunction has been linked to various muscle disorders. It has been reported that a diverse collection of extracellular matrix constituents, including decorin, collagen VI, laminin alpha 2, endorepellin, and endostatin, can modulate autophagic signaling pathways. However, the association between autophagy and perlecan in muscle homeostasis remains unclear. The mechanical unloading of perlecan-deficient soleus muscles resulted in significantly decreased wet weights and cross-section fiber area compared with those of control mice. We found that perlecan deficiency in slow-twitch soleus muscles enhanced autophagic activity. This was accompanied by a decrease in autophagic substrates, such as p62, and an increase in LC3II levels. Furthermore, perlecan deficiency caused a reduction in the phosphorylation levels of p70S6k and Akt and increased the phosphorylation of AMPK alpha. Our findings suggested that perlecan inhibits the autophagic process through the activation of the mTORC1 pathway. This autophagic response may be a novel target for enhancing the efficacy of skeletal muscle atrophy treatment. (C) 2015 Published by Elsevier B.V.
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