Journal
INDIAN JOURNAL OF MICROBIOLOGY
Volume 54, Issue 3, Pages 315-322Publisher
SPRINGER
DOI: 10.1007/s12088-014-0446-z
Keywords
Acinetobacter calcoaceticus; Wheat rhizosphere; NMR; Acinetobactin-like siderophore; Fusarium oxysporum
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Funding
- Department of Biotechnology (DBT), Govt. of India, New Delhi [BT/PR6454/AGR/05/302/2005]
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Acinetobacter calcoaceticus HIRFA32 from wheat rhizosphere produced catecholate type of siderophore with optimum siderophore (ca. 92 % siderophore units) in succinic acid medium without FeSO4 at 28 A degrees C and 24 h of incubation. HPLC purified siderophore appeared as pale yellow crystals with molecular weight [M+1] m/z 347.18 estimated by LCMS. The structure elucidated by H-1 NMR, C-13 NMR, HMQC, HMBC, NOESY and decoupling studies, revealed that siderophore composed of 2,3-dihydroxybenzoic acid with hydroxyhistamine and threonine as amino acid subunits. In vitro study demonstrated siderophore mediated mycelium growth inhibition (ca. 46.87 +/- A 0.5 %) of Fusarium oxysporum. This study accounts to first report on biosynthesis of acinetobactin-like siderophore by the rhizospheric strain of A. calcoaceticus and its significance in inhibition of F. oxysporum.
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