Journal
INDIAN JOURNAL OF MICROBIOLOGY
Volume 53, Issue 3, Pages 370-375Publisher
SPRINGER
DOI: 10.1007/s12088-013-0368-1
Keywords
Porcine reproductive and respiratory syndrome virus; GP5a; Purification; Expression
Categories
Funding
- National Natural Sciences Foundation of China [31272564, 31201876]
- NSFC-Guangdong of China [U0931003]
- Modern Agricultural Industry Technology System [CARS-36]
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Porcine reproductive and respiratory syndrome (PRRS) is considered one of the most important infectious diseases to affect the swine industry and characterized by reproductive failure in late term gestation in sows and respiratory disease in pigs of all ages. The GP5a gene, encoding RNA-dependent RNA polymerase, is generally regarded as fairly conserved when compared to other viral proteins. It plays an important role in the process of duplication and transcription carried out by Porcine reproductive and respiratory syndrome virus (PRRSV). We firstly expressed and purified the GP5a protein of PRRSV. This provides a good method for the purification of expressed proteins and the preparation of the corresponding antibodies.
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