Journal
INDIAN JOURNAL OF MICROBIOLOGY
Volume 53, Issue 1, Pages 77-83Publisher
SPRINGER
DOI: 10.1007/s12088-012-0294-7
Keywords
Bacillus thuringiensis; Dark-fermentation; Hydrogen; Polyhydroxyalkanoates; Levulinic acid
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Funding
- CSIR-Institute of Genomics and Integrative Biology
- Department of Biotechnology, Government of India
- UGC
- CSIR
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Integrative processes for the production of bioenergy and biopolymers are gaining importance in recent years as alternatives to fossil fuels and synthetic plastics. In the present study, Bacillus thuringiensis strain EGU45 has been used to generate hydrogen (H-2), polyhydroxybutyrate (PHB) and new co-polymers (NP). Under batch culture conditions with 250 ml synthetic media, B. thuringiensis EGU45 produced up to 0.58 mol H-2/mol of glucose. Effluent from the H-2 production stage was incubated under shaking conditions leading to the production of PHB up to 95 mg/l along with NP of levulinic acid up to 190 mg/l. A twofold to fourfold enhancement in PHB and up to 1.5 fold increase in NP yields was observed on synthetic medium (mixture of M-9+GM-2 medium in 1:1 ratio) containing at 1-2 % glucose concentration. The novelty of this work lies in developing modified physiological conditions, which induce bacterial culture to produce NP.
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