Journal
INDIAN JOURNAL OF MEDICAL MICROBIOLOGY
Volume 29, Issue 3, Pages 258-261Publisher
ELSEVIER
DOI: 10.4103/0255-0857.83909
Keywords
Extended spectrum beta-lactamases; Providencia stuartii; TEM-116; tunisia
Categories
Funding
- Tunisian Ministry of Higher Education, Scientific Research and Technology
Ask authors/readers for more resources
Purpose: To study the resistance to third-generation cephalosporins in Providencia stuartii strain isolated from hospitalized patient in Tunisia and to identify the responsible genes Materials and Methods: This strain was analysed by PCR and sequencing to identify the genes responsible for the beta-lactamase resistance phenotypes. The transferability of the phenotypes was tested by conjugation to Escherichia coli J53. The isoelectric point was determinate by isoelectrofocalisation. Results: This resistance was carried by a 60 kb plasmid that encoded a beta-lactamase with a pI of 5.4. This beta-lactamase revealed identity with the blaTEM-1 gene encoding the TEM-1 beta-lactamase, except for a replacement of the Val residue at position 84 by Ile, and the Ala residue at position 184 by Val. These two mutations were encountered in TEM-116 beta-lactamase. Conclusion: This study demonstrates the first description of TEM-116 in the P. stuartii species in the world and the first one in a Tunisian hospital.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available