Journal
IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-PLANT
Volume 50, Issue 1, Pages 92-98Publisher
SPRINGER
DOI: 10.1007/s11627-013-9540-x
Keywords
Plant regeneration; Cell suspension; Protoplast culture; Populus x beijingensis
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Funding
- Forestry Public Benefit Research Foundation [201004009]
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A protocol for plant regeneration from cell suspension-derived protoplasts of Populus x beijingensis is described. Protoplasts were isolated from cell suspension cultures 6 d after subculture and further cultured in liquid NH4NO3-free Murashige and Skoog (MS) medium supplemented with 0.6 M glucose, 9.05 mu M 2,4-dichlorophenoxyacetic acid, and 0.89 mu M 6-benzyladenine at a density of 2 x 10(5) protoplasts per milliliter. The initial plating efficiency and final plating efficiency recorded after 10 and 30 d reached 33.7 and 1.07%, respectively. The proliferated calli transferred to regeneration medium supplemented with 2.22 mu M 6-benzyladenine and 0.54 mu M alpha-naphthaleneacetic acid gave the highest rate of shoot formation (44.4%). All protoplast-derived shoots were able to form roots on half-strength MS medium supplemented with 2.46 mu M indole-3-butyric acid.
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