Combination of Adoptive Cell Transfer and Antibody Injection Can Eradicate Established Tumors in Mice-An in vivo study using anti-OX40mAb, anti-CD25mAb and anti-CTLA4mAb-

Boosting an effective immune response against established tumors remains a difficult challenge. This study shows the combination of 1) adoptive cell transfer using CD25 depleted splenocytes co-cultured with irradiated tumor cells, and 2) antibody injection therapy using CTLA4 blockade, the elimination of Treg and OX40, which together could eradicate an established MethA tumor in over 50% of the BALB/c mice. Each element of the protocol was shown to be necessary, as elimination of any factor except anti-CD25 antibody injection failed to eradicate the tumor.