Journal
IMMUNOLOGY LETTERS
Volume 148, Issue 1, Pages 34-38Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.imlet.2012.07.006
Keywords
Melanoma; Tumor immunity; Exosomes; Cytokine profile
Categories
Funding
- National Science Fund of Hungary OTKA [PD 84064, OTKA K 81180, GOP-1.1.1-11-2011-0003, TAMOP-4.2.2/B-10/1-2010-0012]
- Hungarian Academy of Sciences
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To clarify controversies in the literature of the field, we have purified and characterized B16F1 melanoma cell derived exosomes (mcd-exosomes) then we attempted to dissect their immunological activities. We tested how mcd-exosomes influence CD4+T cell proliferation induced by bone marrow derived dendritic cells; we quantified NF-kappa B activation in mature macrophages stimulated with mcd-exosomes, and we compared the cytokine profile of LPS-stimulated, IL-4 induced, and mcd-exosome treated macrophages. We observed that mcd-exosomes helped the maturation of dendritic cells, enhancing T cell proliferation induced by the treated dendritic cells. The exosomes also activated macrophages, as measured by NF-kappa B activation. The cytokine and chemokine profile of macrophages treated with tumor cell derived exosomes showed marked differences from those induced by either LPS or IL-4, and it suggested that exosomes may play a role in the tumor progression and metastasis formation through supporting tumor immune escape mechanisms. (C) 2012 Elsevier B.V. All rights reserved.
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