Journal
IMMUNOLOGY LETTERS
Volume 131, Issue 1, Pages 89-96Publisher
ELSEVIER
DOI: 10.1016/j.imlet.2010.02.009
Keywords
Ecto-sialyltransferase; Sialic acid; Dendritic cell; Endocytosis
Categories
Funding
- Fundacao para a Ciencia e Tecnologia [PTDC/SAU-MII/67561/2006, SFRH/BPD/21619/2005]
- Fundação para a Ciência e a Tecnologia [SFRH/BPD/21619/2005, PTDC/SAU-MII/67561/2006] Funding Source: FCT
Ask authors/readers for more resources
Human monocyte-derived dendritic cells (mo-DCs) express highly sialylated structures, with recognized but poorly understood function in maturation, immunogenicity and endocytosis capacity. We have previously shown that mo-DCs surface sialylation is changeable upon different stimuli, which led us to hypothesise the existence of cell surface (non-intracellular) sialyltransferases, rapidly restoring or altering mo-DC surface sialylation, thus modulating specific functions. Here, we demonstrate that, in the presence of exogenous CMP-Neu5Ac, mo-DCs incorporate considerable amounts of sialic acids into cell surface, predominantly when mo-DCs were previously desialylated or matured. This is a genuine sialyltransferase activity, confirmed by specific inhibition assays, which is not influenced by secreted enzymes. Functionally, the ecto-sialyltransferase activity causes a significant down-regulation of mo-DCs endocytic capacity, without affecting the maturation state. These findings suggest that ecto-sialyltransferases participate in a dynamic control of mo-DC sialylation, with functional repercussions. This activity is possibly related with specific physiological and pathological conditions, as inflammation and infection, contributing to protection and homeostasis regulation. (C) 2010 Elsevier B.V. All rights reserved.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available