Journal
IMMUNOLOGY
Volume 125, Issue 4, Pages 441-449Publisher
WILEY
DOI: 10.1111/j.1365-2567.2008.02989.x
Keywords
flow cytometry; T cells; tetramers; fluorochromes; data analysis
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Funding
- Intramural NIH HHS [Z99 AI999999] Funding Source: Medline
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Multiparameter flow cytometry has matured tremendously since the 1990s, giving rise to a technology that allows us to study the immune system in unprecedented detail. In this article, we review the development of hardware, reagents, and data analysis tools for multiparameter flow cytometry and discuss future advances in the field. Finally, we highlight new applications that use this technology to reveal previously unappreciated aspects of cell biology and immunity.
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