Journal
IMMUNOLOGICAL INVESTIGATIONS
Volume 38, Issue 2, Pages 132-152Publisher
TAYLOR & FRANCIS INC
DOI: 10.1080/08820130802667499
Keywords
DNFB; Macrophages; MIP-2; ROS
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Funding
- Next Generation Growth Engine Program of Korea [F104AC010002-06A0301-00230]
- Korea Research Foundation Grant [KRF-2005-005-J15001, KRF-2005-070-C00091]
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The skin sensitizer 2,4-dinitrofluorobenzene (DNFB) provokes delayed hypersensitivity responses as a result of topical application to the skin. Here, we demonstrate that DNFB modifies proteins in RAW 264.7 cells and skin tissues in NC/Nga mice; we also show the functional involvement of DNFB-induced modification of cellular proteins in the DNFB-induced macrophage inflammatory protein (MIP)-2 gene expression in RAW 264.7 cells. In addition, we demonstrate that DNFB strongly induces reactive oxygen species (ROS) production. Our RT-PCR analysis and reporter gene assays reveal that the DNFB-induced intracellular ROS production is necessary for MIP-2 gene expression by DNFB. We observed that the vitamin C and chemical oxidant scavenger N-acetyl-cysteine have an inhibitory effect on the generation of ROS, the activation of MAP kinase pathways, and the MIP-2 gene expression in DNFB-treated RAW 264.7 cells. These results provide insight into the mechanisms involved in DNFB-induced contact hypersensitivity.
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