Journal
IMMUNOLOGIC RESEARCH
Volume 58, Issue 2-3, Pages 374-377Publisher
HUMANA PRESS INC
DOI: 10.1007/s12026-014-8511-6
Keywords
Monocytes; Inflammatory dendritic cells; CD209; Antibody-conjugated toxin; Saporin
Categories
Funding
- NCI NIH HHS [F31 CA142272] Funding Source: Medline
- NHLBI NIH HHS [P01 HL075462] Funding Source: Medline
- NIDDK NIH HHS [R01 DK082537, R01 DK096038] Funding Source: Medline
Ask authors/readers for more resources
Monocytes rapidly infiltrate inflamed tissues and differentiate into CD209(+) inflammatory dendritic cells (DCs) that promote robust immunity or, if unregulated, inflammatory disease. Previous studies in experimental animal models indicate that inflammatory DC depletion through systemic elimination of their monocyte precursors with clodronate-loaded liposomes ameliorates the development of psoriasis and other diseases. However, translation of systemic monocyte depletion strategies is difficult due to the importance of monocytes during homeostasis and infection clearance. Here, we describe a strategy that avoids the monocyte intermediates to deplete inflammatory DCs through antibody-loaded toxin. Mice with an abundance of inflammatory DCs as a consequence of lipopolysaccharide exposure were treated with anti-CD209 antibody conjugated to saporin, a potent ribosome inactivator. The results demonstrate depletion of CD209(+) DCs. This strategy could prove useful for the targeted reduction of inflammatory DCs in disease.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available