Journal
IMMUNOLOGIC RESEARCH
Volume 56, Issue 1, Pages 32-43Publisher
HUMANA PRESS INC
DOI: 10.1007/s12026-012-8377-4
Keywords
Mast cell; Influenza A; Myxovirus; Innate immunity; Antiviral; Hemagglutinin
Categories
Funding
- Canadian Institutes of Health Research (CIHR)
- CIHR
Ask authors/readers for more resources
Mast cells are important in innate immunity and protective against certain bacterial infections. However, there is limited evidence that mast cells respond to viruses. As mast cells are abundant in mucosal tissues of the lung, they are in a prime location to detect and respond to influenza virus. In this study, we characterized for the first time the replication cycle of influenza A virus in human mast cells by measuring influenza A virus transcription, RNA replication, protein synthesis, and formation of infectious virus as compared to the replication cycle in epithelial cells. We detected the presence of influenza A viral genomic RNA transcription, replication, and protein synthesis in human mast cells and epithelial cells. However, there was no significant release of infectious influenza A virus from mast cells, whereas epithelial cells produce similar to 100-fold virus compared with the inoculating dose. We confirmed that influenza A virus infects human mast cells, begins to replicate, but the production of new virus is aborted. Thus, mast cells may lack critical factors essential for productive infection or there are intrinsic or inducible anti-influenza A mechanisms in mast cells.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available