Journal
IMMUNOGENETICS
Volume 62, Issue 11-12, Pages 773-780Publisher
SPRINGER
DOI: 10.1007/s00251-010-0481-9
Keywords
Mauritian cynomolgus macaque; MHC; Pyrosequencing; Macaca fasicularis
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Funding
- US National Institute of Allergy and Infectious Disease at the Wisconsin National Primate Research Center [HHSN266200400088C/N01-AI-40088]
- National Center for Research Resources, a component of the National Institutes of Health [P51 RR000167]
- Research Facilities Improvement Program [RR15459-01, RR020141-01]
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Major histocompatibility complex (MHC) class I alleles of nonhuman primates have been associated with disease susceptibility, resistance, and resolution. Here, using high-resolution pyrosequencing, we characterized MHC class I transcripts expressed in Mauritian cynomolgus macaques (MCM), a nonhuman primate population with restricted MHC diversity. Using this approach, we identified 67 distinct MHC class I transcripts encoded by the seven most frequent MCM MHC class I haplotypes, 40 (60%) of which span the complete open reading frames. These results double the number of MHC class I sequences previously defined by cloning and Sanger sequencing of cDNA-PCR products and provide a rapid, high-throughput, and economical method for MHC characterization. Overall, this approach significantly expanded our knowledge of MCM haplotypes and will facilitate future studies on disease pathogenesis and protective cellular immunity.
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