Journal
IMMUNOGENETICS
Volume 62, Issue 1, Pages 23-29Publisher
SPRINGER
DOI: 10.1007/s00251-009-0407-6
Keywords
Zebrafish; T cell receptor; VDJ rearrangement; Repertoire diversity
Categories
Funding
- National Institutes of Health (NIH) NICHD [K12 HD001410, 5 K08 HD53350]
- Alex's Lemonade Stand Young Investigator
- Children's Health Research Center at the University of Utah
- Primary Children's Medical Center Foundation
- NIH NIDDK [DK007115]
- NIH NIAID [R21 AI079784]
- Huntsman Cancer Foundation
- Huntsman Cancer Institute [P30 CA042014]
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Zebrafish (Danio rerio) has become an increasingly important model for immunological study. Its immune system is remarkably similar to that of mammals and includes both the adaptive and innate branches. Zebrafish T cells express functional T cell receptors (TCR), and all four TCR loci are present within the genome. Using 5'-rapid amplification of cDNA ends, we cloned and sequenced zebrafish TCR beta transcripts. TCR beta VDJ coding joints demonstrate conservation of mechanisms used by other vertebrate species to increase junctional diversity. Using the sequences obtained, along with previously published data, we comprehensively annotated the zebrafish TCR beta locus. Overall, organization of the locus resembles that seen in mammals. There are 51 V segments, a single D segment, 27 J beta 1 segments, a single J beta 2 segment, and two constant regions. This description of the zebrafish TCR beta locus has the potential to enhance immunological research in zebrafish and further our understanding of mammalian TCR repertoire generation.
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