Journal
IMMUNOBIOLOGY
Volume 216, Issue 3, Pages 351-357Publisher
ELSEVIER GMBH
DOI: 10.1016/j.imbio.2010.07.009
Keywords
Bacterial biofilms; Complement; IgG; Neutrophils; Oxygen radicals; Phagocytosis; Staphylococcus aureus
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Funding
- Deutsche Forschungsgemeinschaft [WA1623/1-5]
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Bacterial biofilms are increasingly recognised as a major cause of persistent infection and destructive inflammatory processes. In patients with biofilm infection, massive infiltration of leukocytes, particularly polymorphonuclear neutrophils is seen, and previous in vitro studies showed that PMN were able to phagocytose Staphylococcus aureus biofilms. We now addressed the question whether opsonisation of biofilms with immunoglobulin G and complement enhances the efficiency of phagocytosis, as it has been shown for free-living planktonic bacteria and other particulate materials. We found that incubation of biofilms with normal human serum resulted in IgG binding and in complement activation with deposits on the biofilm of C3bi. This opsonisation, however, did not affect the adherence of PMN to the biofilms nor did it enhance degranulation or phagocytosis. The clearance of biofilms, however, was increased, and the oxygen radical production by the PMN depended critically on the coating of biofilms with IgG. (C) 2010 Elsevier GmbH. All rights reserved.
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