Journal
IMMUNITY
Volume 38, Issue 3, Pages 461-474Publisher
CELL PRESS
DOI: 10.1016/j.immuni.2012.11.019
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Funding
- Cancer Research UK
- Royal Society Wolfson Research Merit Award
- Human Frontier Science Program
- Cancer Research UK [15691] Funding Source: researchfish
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A key role is emerging for the cytoskeleton in coordinating receptor signaling, although the underlying molecular requirements remain unclear. Here we show that cytoskeleton disruption triggered signaling requiring not only the B cell receptor (BCR), but also the coreceptor CD19 and tetraspanin CD81, thus providing a mechanism for signal amplification upon surface-bound antigen stimulation. By using superresolution microscopy, we demonstrated that endogenous IgM, IgD, and CD19 exhibited distinct nanoscale organization within the plasma membrane of primary B cells. Upon stimulation, we detect a local convergence of receptors, although their global organization was not dramatically altered. Thus, we postulate that cytoskeleton reorganization releases BCR nanoclusters, which can interact with CD19 held in place by the tetraspanin network. These results not only suggest that receptor compartmentalization regulates antigen-induced activation but also imply a potential role for CD19 in mediating ligand-independent tonic'' BCR signaling necessary for B cell survival.
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