Journal
MATERIALS SCIENCE & ENGINEERING C-MATERIALS FOR BIOLOGICAL APPLICATIONS
Volume 48, Issue -, Pages 287-293Publisher
ELSEVIER
DOI: 10.1016/j.msec.2014.12.020
Keywords
Biosensor; Glucose oxidase; Pyrrole; Immobilization; Chitosan
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A film of chitosan-polypyrrole-gold nanoparticles was fabricated by in-situ chemical synthesis method and its application in glucose biosensor was investigated. The obtained biosensor exhibited a high and reproducible sensitivity of 0.58 mu A/mM, response time similar to 4 s, linear dynamic range from 1 to 20 mM, correlation coefficient of R-2 0.9981, and limit of detection (LOD), based on S/N ratio (S/N = 3) of 0.068 mM. A value of 1.83 mM for the apparent Michaelis-Menten constant was obtained. The resulting bio-nanocomposite provided a suitable environment for the enzyme to retain its bioactivity at considerably extreme conditions, and the decorated gold nanoparticles in the bio-nanocomposite offer good affinity to enzyme. (C) 2014 Published by Elsevier B.V.
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