Journal
MATERIALS SCIENCE & ENGINEERING C-MATERIALS FOR BIOLOGICAL APPLICATIONS
Volume 50, Issue -, Pages 226-233Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.msec.2015.02.005
Keywords
Silk fibroin; Molecular weight; Salting out; Microspheres; Ionic strength
Categories
Funding
- National Natural Science Foundation of China [91027039, 51373110, 51203108]
- Natural Science Foundation of Jiangsu Province of China [BK2011355]
- Natural Science Foundation of the Jiangsu Higher Education Institutions of China [11KJB430011, 10KJA540046]
- Nanotechnology Foundation of Suzhou [ZXG2013037]
- China Postdoctoral Science Foundation [2013M531403]
- Priority Academic Program Development ofJiangsu Higher Education Institutions (PAPD)
- Qing Lan Project for Excellent Scientific and Technological Innovation Team ofJiangsu Province
- Jiangsu Scientific and Technological Innovation Team
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Silk fibroin (SF) microspheres were produced by salting out SF solution via the addition of potassium phosphate buffer solution (K2HPO4-KH2PO4). The morphology, size and polydispersity of SF microspheres were adjusted by changing the molecular weight (MW) distribution and concentration of SF, as well as the ionic strength and pH of the buffer solution. Changing the conditions under which the SF fiber dissolved in the Lithium Boride (LiBr) solution resulted in altering the MW distribution of SF solution. Under optimal salting-out conditions (ionic strength > 0.7 M and pH > 7) and using a smaller and narrower SF MW distribution, SF microspheres with smoother shapes and more uniform sizes were produced. Meanwhile, the size and polydispersity of the microspheres increased when the SF concentration was increased from 0.25 mg/mL to 20 mg/mL. The improved SF microspheres, obtained by altering the distribution of molecular weight, have potential in drug and gene delivery applications. (C) 2015 Elsevier B.V. All rights reserved.
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