Journal
MATERIALS SCIENCE & ENGINEERING C-MATERIALS FOR BIOLOGICAL APPLICATIONS
Volume 55, Issue -, Pages 193-200Publisher
ELSEVIER
DOI: 10.1016/j.msec.2015.05.028
Keywords
Porphyrin; gamma-Fe2O3; One-pot method; Peroxidase; Calorimetric sensor
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Funding
- National Natural Science Foundation of China [21271119]
- Key Laboratory of Photochemical Conversion and Optoelectronic Materials, TIPC, CAS [PCOM 201405]
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Meso-tetrakis(4-carboxyphenyl)-porphyrin-fiinctionalized gamma-Fe2O3 nanoparticles (H2TCPP-gamma-Fe2O3) were successfully prepared by one-pot method under hydrothermal conditions and were found to possess intrinsic peroxidase-like activity. The H2TCPP-gamma-Fe2O3 nanocomposites can catalytically oxidize peroxidase substrate 3,3',5,5'-tetramethylbenzidine (TMB) in the presence of H2O2 to produce a blue color reaction, which can be easily observed by the naked eye. Furthermore, kinetic studies indicate that the H2TCPP-gamma-Fe2O3 nanocomposites have an even higher affinity to TMB than that of the natural enzyme, horseradish peroxidase (HRP). On the basis of the high activity, the reaction provides a simple, sensitive and selective method for colorimetric detection of H2O2 over a range of 10-100 mu M with a minimum detection limit of 1.73 mu M. Moreover, H2TCPP-gamma-Fe2O3/glucose oxidase (GOx)/TMB system provides a novel colorimetric sensor for glucose and shows good response toward glucose detection over a range of 5-25 mu M with a minimum detection limit of 254 mu M. The results indicated that it is a simple, cheap, convenient highly selective, sensitive and easy handling colorimetric assay. Results of a fluorescent probe suggest that the catalase-mimic activity of the H2TCPP-gamma-Fe2O3 nanocomposites effectively catalyze the decomposition of H2O2 into H2O and O-2. (C) 2015 Elsevier B.V. All rights reserved.
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