Journal
MATERIALS SCIENCE AND ENGINEERING C-MATERIALS FOR BIOLOGICAL APPLICATIONS
Volume 53, Issue -, Pages 43-49Publisher
ELSEVIER
DOI: 10.1016/j.msec.2015.04.016
Keywords
Biosensor; WO3; Nanowires with high length-diameter ratio; Direct electron transfer (DET); Nitrite
Categories
Funding
- National Science Foundation of China [51272147]
- Academic Backbone Cultivation Program of Shaanxi University of Science Technology [XSGP201203]
- Graduate Innovation Found of Shaanxi University of Science and Technology
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WO3 nanowires (WO(3)NWs) with high length-diameter ratio have been synthesized through a simple synthetic route without any additive and then used to immobilize hemoglobin (Hb) to fabricate a mediator-free biosensor. The morphology and structure of WO(3)NWs were characterized by scanning electron microscopy, transmission electronic microscopy and X-ray diffraction. Spectroscopic and electrochemical results revealed that WO(3)NWs are an excellent immobilization matrix with biocompatibility for redox protein, affording good protein bioactivity and stability. Meanwhile, due to unique morphology and property of the WO3 nanowires, the direct electron transfer of Hb is facilitated and the prepared biosensors displayed good performance for the detection of nitrite with a wide linear range of 1 to 4200 mu M, as well as an extremely low detection limit of 0.28 mu M. The WO3 nanowires with high length-diameter ratio could be a promising matrix for the fabrication of mediator-free biosensors, and may find wide potential applications in environmental analysis and biomedical detection. (C) 2015 Elsevier B.V. All rights reserved.
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