Journal
MATERIALS SCIENCE & ENGINEERING C-MATERIALS FOR BIOLOGICAL APPLICATIONS
Volume 49, Issue -, Pages 786-792Publisher
ELSEVIER
DOI: 10.1016/j.msec.2015.01.048
Keywords
Arthrobacter creatinolyticus; Urease immobilization; PAN membrane; Cross-linking agent; Potentiometric urea biosensor
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Funding
- Council of Scientific and Industrial Research, Government of India
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The extracellular urease from Arthrobacter creatinotyticus was partially purified by ammonium sulfate precipitation and immobilized on PAN [poly(acrylonitrile-methylmethacrylate-sodium vinylsulfonate)] membrane. The urease immobilized PAN membrane exhibited an activity of 97.92 U/cm(2) under the optimum conditions of 1.0% enzyme concentration, 15% glutaraldehyde, 24 h immobilization time and temperature of 4 degrees C. The changes in surface morphology of the membrane after immobilization were studied by SEM and ATR-FTIR analysis. Immobilized membrane was associated with potentiometric electrode for calibration of biosensor and the results showed a linear response for wide range of urea concentration from 1 to 100 mM. The immobilized urease had good storage stability for a period of 70 days at 4 degrees C and could be effectively reused for 13 cycles. Urease immobilized membrane was also employed in analysis of urea spiked milk samples. (C) 2015 Elsevier B.V. All rights reserved.
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