Journal
MATERIALS SCIENCE & ENGINEERING C-MATERIALS FOR BIOLOGICAL APPLICATIONS
Volume 54, Issue -, Pages 150-157Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.msec.2015.05.006
Keywords
Aspartic acid; Glutamic acid; Hydroxyapatite; Calcium carbonate; Enamel
Categories
Funding
- National Natural Science Foundation of Jilin Province [201015202]
- Medical Scientific Research Foundation of Health and Family Planning Commission of Jilin Province [2014Z064]
- Science and Technology Agency of Jilin Province [20130206056GX]
- Scientific Research Foundation for the Returned Overseas Chinese Scholars, State Education Ministry
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In situ fabrication of carbonated hydroxyapatite (CHA) remineralization layer on an enamel slice was completed in a novel, biomimetic two-step method. First, a CaCO3 layer was synthesized on the surface of demineralized enamel using an acidic amino acid (aspartic acid or glutamate add) as a soft template. Second, at the same concentration of the acidic amino acid, rod-like carbonated hydroxyapatite was produced with the CaCO3 layer as a sacrificial template and a reactant. The morphology, crystallinity and other physicochemical properties of the crystals were characterized using field emission scanning electron microscopy (FESEM), Fourier transform infrared spectrometry (FTIR), X-ray diffraction (XRD) and energy-dispersive X-ray analysis (EDAX), respectively. Acidic amino acid could promote the uniform deposition of hydroxyapatite with rod-like crystals via absorption of phosphate and carbonate ions from the reaction solution. Moreover, compared with hydroxyapatite crystals coated on the enamel when synthesized by a one-step method, the CaCO3 coating that was synthesized in the first step acted as an active bridge layer and sacrificial template. It played a vital role in orienting the artificial coating layer through the template effect The results show that the rod-like carbonated hydroxyapatite crystals grow into bundles, which are similar in size and appearance to prisms in human enamel, when using the two-step method with either aspartic acid or acidic glutamate (20.00 mmol/L). (C) 2015 Elsevier B.V. All rights reserved.
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