Journal
IEEE TRANSACTIONS ON NANOBIOSCIENCE
Volume 11, Issue 4, Pages 317-323Publisher
IEEE-INST ELECTRICAL ELECTRONICS ENGINEERS INC
DOI: 10.1109/TNB.2012.2210442
Keywords
Avidin; biotin; micro-emulsion; synthesis; nanocrystals; ZnS:Mn
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In this research, we mainly focused on the microemulsion synthesis of biotinylated ZnS (zinc sulfide) nanocrystals for avidin recognition. Various samples of Zn(1-x)Mn(X)s with x = 0.0001, 0.007, 0.02, 0.03, 0.055, 0.09 and 0.13, prepared by quaternary W/O (water-in-oil) microemulsion system. Cyclohexane was used as oil, Triton X-100 as surfactant, n-hexanol as a co-surfactant and mercaptoethanol and thioglycolic acid as linking agents. The obtained products were evaluated by commonly techniques such as: scanning electron microscopy (SEM), transmission electron microscopy (TEM), zeta meter for measurement ZP (zeta potential) and fluorescence spectroscopy analyses. The above-experimental results indicated that the optimum doping concentration of Mn was similar to 5.5%. The fluorescence spectra of the doped crystals consist of orange-red emissions. Eventually, this research showed with increasing more than biotin to nanocrystals, no changes were observed in the emission intensity spectra.
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