Journal
IEEE TRANSACTIONS ON MEDICAL IMAGING
Volume 31, Issue 1, Pages 1-15Publisher
IEEE-INST ELECTRICAL ELECTRONICS ENGINEERS INC
DOI: 10.1109/TMI.2011.2161590
Keywords
Artifacts; axis of rotation (AR); biomedical image processing; islets of Langerhans; optical projection tomography (OPT); pancreas; postalignment
Categories
Funding
- Kempe Foundations
- European Commission [CP-IP 228933-2]
- Umea University
- ICREA Funding Source: Custom
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Since it was first presented in 2002, optical projection tomography (OPT) has emerged as a powerful tool for the study of biomedical specimen on the mm to cm scale. In this paper, we present computational tools to further improve OPT image acquisition and tomographic reconstruction. More specifically, these methods provide: semi-automatic and precise positioning of a sample at the axis of rotation and a fast and robust algorithm for determination of postalignment values throughout the specimen as compared to existing methods. These tools are easily integrated for use with current commercial OPT scanners and should also be possible to implement in home made or experimental setups for OPT imaging. They generally contribute to increase acquisition speed and quality of OPT data and thereby significantly simplify and improve a number of three-dimensional and quantitative OPT based assessments.
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