Journal
MARINE DRUGS
Volume 13, Issue 1, Pages 249-266Publisher
MDPI AG
DOI: 10.3390/md13010249
Keywords
Salinispora arenicola; salt concentration; antibiotic; metabolomics; secondary metabolites; liquid chromatography; mass spectrometry; multivariate analysis
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Funding
- University of Queensland International Scholarship
- University of Queensland Research Scholarship (UQRS)
- University of Queensland International Research Tuition Award (UQIRTA)
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An LC-MS-based metabolomics approach was used to characterise the variation in secondary metabolite production due to changes in the salt content of the growth media as well as across different growth periods (incubation times). We used metabolomics as a tool to investigate the production of rifamycins (antibiotics) and other secondary metabolites in the obligate marine actinobacterial species Salinispora arenicola, isolated from Great Barrier Reef (GBR) sponges, at two defined salt concentrations and over three different incubation periods. The results indicated that a 14 day incubation period is optimal for the maximum production of rifamycin B, whereas rifamycin S and W achieve their maximum concentration at 29 days. A chemical profile link between the days of incubation and the salt concentration of the growth medium was shown to exist and reliably represents a critical point for selection of growth medium and harvest time.
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