Journal
IEEE TRANSACTIONS ON BIOMEDICAL ENGINEERING
Volume 58, Issue 3, Pages 805-808Publisher
IEEE-INST ELECTRICAL ELECTRONICS ENGINEERS INC
DOI: 10.1109/TBME.2010.2089054
Keywords
Device; disposable; methicillin resistant Staphylococcus aureus (MRSA); point-of-care testing (POCT); triage; virus
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Funding
- Lawrence Livermore National Laboratory [08-ERD-044, DE-AC52-07NA27344]
- National Institute of Biomedical Imaging and Bioengineering [U54EB007959]
- U.K. Department for Environment, Food and Rural Affairs [SE1124]
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Development of small footprint, disposable, fast, and inexpensive devices for pathogen detection in the field and clinic would benefit human and veterinary medicine by allowing evidence-based responses to future out breaks. We designed and tested an integrated nucleic acid extraction and amplification device employing a loop-mediated isothermal amplification (LAMP) or reverse transcriptase-LAMP assay. Our system provides a screening tool with polymerase-chain-reaction-level sensitivity and specificity for outbreak detection, response, and recovery. Time to result is similar to 90 min. The device utilizes a swab that collects sample and then transfers it to a disc of cellulose-based nucleic acid binding paper. The disc is positioned within a disposable containment tube with a manual loading port. In order to test for the presence of target pathogens, LAMP reagents are loaded through the tube's port into contact with the sample containing cellulose disc. The reagents then are isothermally heated to 63 degrees C for similar to 1 h to achieve sequence-specific target nucleic acid amplification. Due to the presence of a colorimetric dye, amplification induces visible color change in the reagents from purple to blue. As initial demonstrations, we detected methicillin resistant Staphylococcus aureus genomic DNA, as well as recombinant and live foot-and-mouth disease virus.
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