Journal
IEEE TRANSACTIONS ON BIOMEDICAL ENGINEERING
Volume 58, Issue 3, Pages 781-784Publisher
IEEE-INST ELECTRICAL ELECTRONICS ENGINEERS INC
DOI: 10.1109/TBME.2010.2066275
Keywords
Chlamydia trachomatis (CT); lysis; microwave-accelerated metal-enhanced fluorescence (MAMEF)
Categories
Funding
- National Institute of Biomedical Imaging and Bioengineering, NIH [1U54 EB007958-4]
- Institute of Fluorescence, University of Maryland Baltimore County
- Department of Chemistry and Biochemistry, University of Maryland Baltimore County
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An inexpensive technology to both lyse Chlamydia trachomatis (CT) and detect DNA released from CT within 40 s is demonstrated. In a microwave cavity, energy is highly focused using 100-nm gold films with bow-tie structures to lyse CT within 10 s. The ultrafast detection of the released DNA from less than 100 cfu/mL CT is accomplished in an additional 30 s by employing the microwave-accelerated metal-enhanced fluorescence technique. This new release and detect platform technology is a highly attractive alternative method for the lysing of bacteria, DNA extraction, and the fast quantification of bacteria and potentially other pathogenic species and cells as well. Our approach is a significant step forward for the development of a point of care test for CT.
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