4.7 Article

Optoelectronic Tweezers as a Tool for Parallel Single-Cell Manipulation and Stimulation

Journal

Publisher

IEEE-INST ELECTRICAL ELECTRONICS ENGINEERS INC
DOI: 10.1109/TBCAS.2009.2031329

Keywords

Dielectrophoresis; electroporation; lysis; optical manipulation; optoelectronic tweezers

Funding

  1. Center for Cell Control
  2. National Institute of Health Nanomedicine Development Center [PN2 EY018228]
  3. NATIONAL EYE INSTITUTE [PN2EY018228] Funding Source: NIH RePORTER

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Optoelectronic tweezers (OET) is a promising approach for the parallel manipulation of single cells for a variety of biological applications. By combining the manipulation capabilities of OET with other relevant biological techniques (such as cell lysis and electroporation), one can realize a true parallel, single-cell diagnostic and stimulation tool. Here, we demonstrate the utility of the OET device by integrating it onto single-chip systems capable of performing in-situ, electrode-based electroporation/lysis, individual cell, light-induced lysis, and light-induced electroporation.

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