Effect of correction on contrast kinetic model analysis using a reference tissue arterial input function at 7 T

We aimed to investigate the effect of correction on estimation of kinetic parameters from T (1)-weighted dynamic contrast enhanced (DCE) MRI data when a reference-tissue arterial input function (AIF) is used. DCE-MRI data were acquired from seven mice with 4T1 mouse mammary tumors using a double gradient echo sequence at 7 T. The AIF was estimated from a region of interest in the muscle. The extended Tofts model was used to estimate pharmacokinetic parameters in the enhancing part of the tumor, with and without correction of the lesion and AIF. The parameters estimated with correction of both the AIF and lesion time-intensity curve were assumed to be the reference standard. For the whole population, there was significant difference (p < 0.05) in transfer constant (K (trans)) between corrected and not corrected methods, but not in interstitial volume fraction (v (e)). Individually, no significant differences were found in K (trans) and v (e) of four and six tumors, respectively, between the corrected and not corrected methods. In contrast, K (trans) was significantly underestimated, if the correction was not used, in other tumors for which the median K (trans) was larger than 0.4 min(-1). effect on tumors with high K (trans) may not be negligible in kinetic model analysis, even if AIF is estimated from reference tissue where the concentration of contrast agent is relatively low.