Journal
IEEE SENSORS JOURNAL
Volume 14, Issue 9, Pages 3008-3013Publisher
IEEE-INST ELECTRICAL ELECTRONICS ENGINEERS INC
DOI: 10.1109/JSEN.2014.2321167
Keywords
CTC detection; immunomagnetic separation; microfluidics; rare cell detection; tumor cells; size-based filtration
Funding
- National Science Foundation [0925417]
- Purdue Oncological Sciences Center
- Purdue Center for Cancer Research
- Walther Cancer Foundation
- Indiana University Simon Cancer Center
- Directorate For Engineering
- Div Of Electrical, Commun & Cyber Sys [0925417] Funding Source: National Science Foundation
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We report a microchip system based on a combination of immunomagnetic separation, microfluidics, and size-based filtration for high-throughput detection of rare cells. In this system, target cells bind to magnetic beads in vitro and flow parallel to a microchip with flow rates of milliliters/minute. A magnetic field draws the bead-bound cells toward the microchip, which contains apertures that allow passage of unbound beads while trapping the target cells. The cells captured on the chip can be investigated clearly under a microscope and released from the chip for further analysis. We first characterize the system by detecting cancer cell lines (MCF-7 and A549) in culture media. We then demonstrate detection of 100 MCF-7 cells spiked in 7.5 mL of human blood to simulate detection of circulating tumor cells present in cancer patient blood samples. On average, 85% of the spiked cells were detected. We expect this system to be highly useful in a wide variety of clinical as well as other applications that seek rare cells.
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