4.7 Article

A Simple, Low-Cost Double Beam Spectrophotometer for Colorimetric Detection of Nitrite in Seawater

Journal

IEEE SENSORS JOURNAL
Volume 9, Issue 7, Pages 862-869

Publisher

IEEE-INST ELECTRICAL ELECTRONICS ENGINEERS INC
DOI: 10.1109/JSEN.2009.2020659

Keywords

Absorbance measurements; colorimetric detection; nitrite sensor; spectroscopy

Funding

  1. NERC
  2. School of Electronics and Computer Science, University of Southampton, Southampton, U. K
  3. EPSRC [EP/E016774/1] Funding Source: UKRI
  4. NERC [soc010010] Funding Source: UKRI
  5. Engineering and Physical Sciences Research Council [EP/E016774/1] Funding Source: researchfish
  6. Natural Environment Research Council [soc010010] Funding Source: researchfish

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This paper reports on the optical detection system of a prototype nitrite sensor for seawater analysis. The sensor uses a colorimetric Griess reaction that produces an Azo dye with an absorption peak at 540 nm. A simple low-cost double beam spectrophotometer using a green light emitting diode (LED) light source, two integrated photo detectors and lock-in detection is described. A log-ratio amplifier is used to produce an output proportional to absorbance. The optical devices were mounted in a cast resin block to prevent shock and vibration. Experimental results show the detection system has a limit of detection of 0.1 mu M.

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