4.7 Article

Evaluating the Thickness of Multivalent Glycopolymer Brushes for Lectin Binding

Journal

MACROMOLECULAR RAPID COMMUNICATIONS
Volume 36, Issue 16, Pages 1472-1478

Publisher

WILEY-V C H VERLAG GMBH
DOI: 10.1002/marc.201500118

Keywords

atom transfer radical polymerization (ATRP); electrochemical impedance spectroscopy (EIS); glycopolymers; lectins; microfluidics

Funding

  1. German Science Foundation (DFG) [SFB 985]

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Electrochemical impedance spectroscopy (EIS) is applied for investigating binding of lectins to multivalent glycopolymer brushes grafted from interdigital gold microelectrodes. By variation of the measuring frequency, EIS allows simultaneous analysis of binding at different subnanometer distances from the sensor surfaces. In this way, the binding dynamics along the brushes are quantified, giving an idea about the motion of the lectin through the brush layer. Two different brush lengths are investigated, revealing distinct dynamics of lectin binding due to changing topology of the brushes. Moreover, very low K-D values in the nanomolar range are obtained. This unique platform may be used as sophisticated biosensor for detailed investigation of high-affinity protein binding to polymer layers.

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