4.7 Article

Co-Culture of Human Endothelial Cells and Foreskin Fibroblasts on 3D Silk-Fibrin Scaffolds Supports Vascularization

Journal

MACROMOLECULAR BIOSCIENCE
Volume 15, Issue 10, Pages 1433-1446

Publisher

WILEY-V C H VERLAG GMBH
DOI: 10.1002/mabi.201500054

Keywords

fibrin; foreskin fibroblasts; human umbilical vein endothelial cells (HUVECs); silk fibroin; vascularization

Funding

  1. Department of Biotechnology, Government of India
  2. German Academic Exchange Service (DAAD)
  3. Applied Medical Engineering, RWTH, Aachen, Germany
  4. two photon image facility, a core facility of the Interdisciplinary Center for Clinical Research (IZKF) Aachen within the Faculty of Medicine at RWTH Aachen University, Germany

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A successful strategy to enhance the in vivo survival of engineered tissues would be to prevascularize them. In this study, fabricated silk fibroin scaffolds from mulberry and non-mulberry silkworms are investigated and compared for supporting the co-culture of human umbilical vein endothelial cells and human foreskin fibroblasts. Scaffolds are cytocompatible and when combined with fibrin gel support capillary-like structure formation. Density and interconnectivity of the formed structures are found to be better in mulberry scaffolds. ELISA shows that levels of vascular endothelial growth factor (VEGF) released in co-cultures with fibrin gel are significantly higher than in co-cultures without fibrin gel. RT PCR shows an increase in VEGFR2 expression in mulberry scaffolds indicating these scaffolds combined with fibrin provide a suitable microenvironment for the development of capillary-like structures.

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