Journal
MACROMOLECULAR BIOSCIENCE
Volume 16, Issue 2, Pages 199-206Publisher
WILEY-V C H VERLAG GMBH
DOI: 10.1002/mabi.201500273
Keywords
cellular reprogramming; induced pluripotent stem cells; mesenchymal-to-epithelial transition; stemness markers; stiffness of hydrogel
Funding
- National Research Foundation of Korea (NRF) - Ministry of Science, ICT & Future Planning [NRF-2013R1A2A1A09013980]
- Ministry of Education, Science and Technology [NRF-2014R1A6A3A04055123]
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The stiffness of hydrogels has been reported to direct cell fate. Here, we found that the stiffness of hydrogels promotes the reprogramming of mouse embryonic fibroblasts into induced pluripotent stem cells (iPSCs). We prepared cell culture substrates of various stiffnesses (0.1, 1, 4, 10, and 20kPa) using a polyacrylamide hydrogel. We found that culture on a soft hydrogel plays an important role in inducing cellular reprogramming into iPSCs via activation of mesenchymal-to-epithelial transition and enhancement of stemness marker expression. These results suggest that physical signals at the interface between cell and substrate can be used as a potent regulator to promote cell fate changes associated with reprogramming into iPSCs, which may lead to effective and reproducible iPSC-production.
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