Journal
IEEE JOURNAL OF SELECTED TOPICS IN QUANTUM ELECTRONICS
Volume 15, Issue 5, Pages 1344-1350Publisher
IEEE-INST ELECTRICAL ELECTRONICS ENGINEERS INC
DOI: 10.1109/JSTQE.2009.2021533
Keywords
2-D; 3-D; dual-axis confocal (DAC) microscopy; electrostatic actuation; endoscope; endoscopy; en face; fluorescence imaging; high-resolution imaging; in vivo; microelectromechanical systems (MEMS) scanner; microscope; mosaicing; near-infrared fluorescence imaging; real-time imaging; self-alignment; vertical comb actuators
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Funding
- National Institutes of Health [U54 CA105296, R33 CA109988]
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We demonstrate a fast miniature microelectromechanical-system-based near-infrared fluorescence dual-axis confocal microscope in a 10-mm-diameter package for 3-D imaging in both ex vivo and in vivo samples. The miniature microscope, while in contact with the targeted tissue, can reveal subsurface structure or anatomy as deep as 300 mu m. The lateral and axial resolutions are 5 and 7 mu m, respectively. Real-time en face mosaicing image of in vivo human skin is demonstrated to enlarge the overall FOV to be over 3 mm at acquisition frame rate of 5 frames/s.
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