Journal
LWT-FOOD SCIENCE AND TECHNOLOGY
Volume 61, Issue 1, Pages 25-32Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.lwt.2014.11.033
Keywords
Grape stems; Polyphenols; HPLC-DAD-ESI-MSn; Antibacterial activity; Intestinal pathogens
Categories
Funding
- national funds from FCT - Portuguese Foundation for Science and Technology [PEst-OE/AGR/UI4033/2014]
- Project INNOFOOD - INNovation in the FOOD sector through the valorization of food and agro-food by-products [NORTE-07-0124-FEDER-0000029]
- Portuguese Foundation for Science and Technology [Carla Dias-SFRH/BD/90120/2012, Alfredo Aires-SFRH/BPD/65029/2009]
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Winemaking industry produces huge amounts of organic residues including grape pomace, grape stems and wine lees, with deleterious impact on the environment and concerning the sustainability of this socio-economic activity, being grape stem the less explored material. The content in phytochemicals of grape and vinification residues as well as the antimicrobial efficiency described for the identified compounds, prompted us to the evaluate the capacity of grape stem's extracts to inhibit the growth of digestive pathogens, and therefore, their potential as a functional ingredient that could prevent microbial disturbance. The phytochemical composition was determined by HPLC-DAD-ESI-MSn and was correlated with the antimicrobial activity in vitro, against Gram positive (Listeria monocytogenes, Staphylococcus aureus and Enterococcus faecalis) and Gram negative (Pseudomonas aeruginosa, Escherichia coli and Klebsiella pneumoniae) digestive pathogens. Antimicrobial activity was determined by the assessment of the Minimum Inhibitory Concentration and the capacity of grape stem's extracts to inhibit microbial growth. The correlation analyses performed pointed out kaempferol-3-O-rutinoside, kaempferol-3-O-glucoside and caftaric acid as compounds responsible for the antimicrobial effect. The confirmation of the inhibitory effect grape stem's extracts on digestive pathogens in vitro encourages further evaluations in vivo on their capacity to prevent the disturbance of intestinal homeostasis. (C) 2014 Elsevier Ltd. All rights reserved.
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