Preparation of potent antioxidant peptide from edible part of shortclub cuttlefish against radical mediated lipid and DNA damage

In this current investigation, the antioxidant peptides were derived from marine Sepia brevimana mantle by trypsin, alpha-chymotrypsin and pepsin for 12 h hydrolysis. The active peptides were found in the hydrolysates of trypsin and alpha-chymotrypsin at the 7th hour and pepsin at the 8th hour. The highest activity was found in trypsin hydrolysate compared to others using various radical scavenging and metal ions transition assays, further the hydrophobic amino acids were observed in more quantity along with other amino acids. Active peptide was purified by consecutive chromatographic techniques and evaluated by DPPH (38.81 +/- 1.07%) and reducing power assays (0.478 +/- 0.03). The purified peptide exhibited significant inhibition for the linoleic acid auto-oxidation in the model system, protective effect on DNA damage due to hydroxyl radical induction and was non-toxic even at higher concentration. The mass and sequence of the peptide was found to be 679.5 Da and Ile/Leu-Asn-Ile/Leu-Cys-Cys-Asn, respectively. The current results suggested that cuttlefish peptide could be used as natural antioxidant in enhancing antioxidant properties of functional foods and in preventing oxidation reactions in food processing. (C) 2015 Elsevier Ltd. All rights reserved.