Journal
HYPERTENSION RESEARCH
Volume 34, Issue 12, Pages 1283-1287Publisher
NATURE PUBLISHING GROUP
DOI: 10.1038/hr.2011.119
Keywords
aldosterone; glucocorticoid response element; mineralocorticoid receptor; osteopontin
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Funding
- Ministry of Education, Culture, Sports, Science and Technology of Japan, [22590823, 17659229]
- Sankyo Foundation of Life Science
- Fugaku Trust for Medical Research
- Grants-in-Aid for Scientific Research [17659229, 22590823, 22590822] Funding Source: KAKEN
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Osteopontin (OPN) is known to be one of the cytokines that is involved in the vascular inflammation caused by aldosterone (Aldo). Previous reports have shown that Aldo increases OPN transcripts, and the mechanisms for this remain to be clarified. In this study, we investigated how Aldo increases OPN transcripts in the vascular smooth muscle cells of rats. Aldosterone increased OPN transcripts time-dependently as well as dose-dependently. This increase was diminished by eplerenone, a mineralocorticoid receptor (MR) antagonist. Luciferase promoter assays showed that the OPN promoter deleted to the -1599 site retained the same promoting ability as the full-length OPN promoter when stimulated by 10(-7) M Aldo, but the promoter deleted to the -1300 site lost the promoting ability. A glucocorticoid response element (GRE) is located in that deleted region. Luciferase assays of a mutated promoter without the GRE lost the luciferase upregulation, although mutated promoters with the deletion of other consensus sites maintained the promoter activity. The binding of the Aldo-MR complex to the GRE fragment was confirmed by an electrophoretic-mobility shift assay. This is the first report showing that Aldo regulates the transcriptional levels of OPN and inflammatory responses in the vasculature through a specific GRE site in the OPN promoter region. Hypertension Research (2011) 34, 1283-1287; doi:10.1038/hr.2011.119; published online 4 August 2011
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