Journal
LIFE SCIENCES
Volume 124, Issue -, Pages 50-55Publisher
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.lfs.2014.12.020
Keywords
Hypoxia-inducible factor-1 alpha; Vascular endothelial growth factor; Aquaporin-1; One-lung ventilation; Acute lung injury
Funding
- National Natural Science Foundation of China [81460016]
- Natural Science Foundation of Guangxi Province of China [2014GXNSFDA118026]
- Health Bureau of Guangxi Province of China [S201418-05]
Ask authors/readers for more resources
Aims: One-lung ventilation (OLV) is a standard practice in thoracic surgery. However, OLV can give rise to arterial hypoxemia. Hypoxia-inducible transcription factor-1 alpha (HIF-1 alpha), vascular endothelial growth factor (VEGF) and aquaporin-1 (AQP-1) may be involved in arterial hypoxemia and contribute to cellular injury. Therefore, in the present study, these moieties were investigated in an OLV rat model. Main methods: Forty Sprague-Dawley (S-D) rats were randomly divided into four groups: right lung mechanical ventilation for 0.5 h (Group A); 1 h (Group B); 2 h (Group C) and mechanical ventilation of both lungs (control group). Rat lung tissue was examined using electron microscopy. Serum and lung tissue levels of VEGF were measured by ELISA, Western blot analyses were used to detect the protein expression of HIP-la and immunohistochemical staining and real-time polymerase chain reaction (PCR) were performed to examine protein expression and gene levels, respectively, of VEGF and AQP-1 after hypoxia. Key findings: Electron microscopy revealed that increased duration of OLV was correlated with greater destruction of the non-ventilated lung. The protein expression of HIF-1 alpha was significantly increased in the non-ventilated lungs of the experimental hypoxia groups (A-C) compared to the control group, whereas VEGF and AQP-1 protein expression and gene levels were decreased in the non-ventilated lungs of the hypoxia groups (A-C) compared to the control group. Significance: The OLV caused hypoxia in the non-ventilated lung and subsequent injury. The altered expression of HIP-1 alpha, VEGF, and AQP-1 may be involved in the pathological process of lung injury caused by hypoxia. (C) 2015 Elsevier Inc. All rights reserved.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available